Journal: Cancers

Article Title: Inhibition of Tumor Microenvironment Cytokine Signaling Sensitizes Ovarian Cancer Cells to Antiestrogen Therapy

doi: 10.3390/cancers14194675

Figure Lengend Snippet: Letrozole and ruxolitinib demonstrate synergy in ovarian cancer cell lines. ERα-high ovarian cancer cell lines OVCAR3 and OVCAR8 were treated with a range of doses of ruxolitinib (Rux) and letrozole (Let) at ratio of 1:2 and cell viability quantified by MTT assay. The combination index (CI) was determined using Compusyn applying the Chou-Talalay method. ( A ) The CI relative to the fraction affected (Fa) is shown for OVCAR3 and OVCAR8. CI at 75% inhibition for each cell line is shown, with values < 1 indicating synergy. ( B ) Colony formation assay for OVCAR8 and graphical representation demonstrating synergy in the colony formation assay.

Article Snippet: Cell viability was determined with the MTT Cell Viability Assay Kit (Biotium, Fremont, CA, USA, Cat. No.30006).

Techniques: MTT Assay, Inhibition, Colony Assay

Journal: Cells

Article Title: Anti-Inflammatory Extract from Soil Algae Chromochloris zofingiensis Targeting TNFR/NF-κB Signaling at Different Levels

doi: 10.3390/cells11091407

Figure Lengend Snippet: Inhibitory effects of NAE_2022C on TNFα- and UVB-induced inflammatory responses. ( A ) Brightfield micrographs of C. zofingiensis cultivated at standard growth conditions (stage 1; Upper) and after nitrogen deprivation stress (stage 2; Lower). Scale bar, 10 μm. ( B ) NAE_2022C inhibited TNFα- but not UVB-induced NF-κB responses. HaCaT cells were incubated with 100 nM DM, 750 nM WFA or 10 µg mL −1 NAE_2022C for 1 h and subsequently stimulated with 0.75 ng mL −1 TNFα or irradiated with 0.15 J/cm 2 UVB. The luciferase activity was measured in cell lysates 6 or 24 h post-stimulation, respectively (left y-axis). The cell viability was evaluated using a resazurin assay and shown as orange squares (right y-axis). Data are presented as mean ± SD ( n = 3). ( C ) The suppression of TNFα-induced pro-inflammatory mediators in reconstituted human epidermis (epiCS) by NAE_2022C. The release of cytokines and chemokines was determined in the medium after the treatment of epiCS with 0.75 ng mL −1 TNFα for 24 h (MCP1, RANTES and TNFα) and 48 h (CXCL10) using the Luminex Assay. Data represent the mean ± SD of at least three independent biological replicates. * p < 0.05 vs. samples treated with DMSO and TNFα.

Article Snippet: The cytotoxicity of algae extracts was evaluated using the Resazurin Cell Viability Kit (Cell Signaling Technology Europe B.V., Frankfurt am Main, Germany) and CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega GmbH, Walldorf, Austria) according to the manufacturers’ protocols.

Techniques: Incubation, Irradiation, Luciferase, Activity Assay, Resazurin Assay, Luminex